Liu, Ying
- University of Copenhagen
Research article2019Peer reviewedOpen access
High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato
Johansen, Ida Elisabeth; Liu, Ying; Jorgensen, Bodil; Bennett, Eric Paul; Andreasson, Erik; Nielsen, Kare L.; Blennow, Andreas; Petersen, Bent Larsen
Abstract
CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.
Keywords
Nuclear RNA gene; guide RNA; genome; expression; sequence; Arabidopsis; prediction; resistance; mutation; cloning
Published in
Scientific Reports
2019, volume: 9, article number: 17715
SLU Authors
Andreasson, Erik
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Plant Biotechnology
Publication identifier
- DOI: https://doi.org/10.1038/s41598-019-54126
Permanent link to this page (URI)
https://res.slu.se/id/publ/102773