Sun, Ren
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
Thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK) are required for the activation of thymidine and deoxycytidine analogs used in antiviral and anticancer therapies. Many anticancer drugs cause oxidative stress, and the rise of GSSG and other reactive oxygen species may lead to alteration in gene expression, protein, nucleic acids and lipid modifications. Here, we investigated the effects of oxidative stress and nucleoside analog on the expression and activity of TK1 and dCK. Treatment with GSSG resulted in glutathionylation of dCK and dGK but not TK1 and Dm-dNK, and glutathionylation led to increased dCK activity but decreased dGK activity. Treatment with hydrogen peroxide resulted in induction of TK1, however, the TK1 activity did not correlate with TK1 protein levels, indicating that TK1 protein was inactive. The cellular expression of dCK, however, was reduced but dCK activity was not affected at concentration
Keywords
Deoxycytidine kinase; doxorubicin; nucleoside analogs; oxidative stress; thymidine kinase 1
Published in
Nucleosides, Nucleotides and Nucleic Acids
2020, volume: 39, number: 10-12, pages: 1347-1358
Publisher: TAYLOR & FRANCIS INC
SLU Authors
Eriksson, Staffan
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Wang, Liya
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Associated SLU-program
AMR: Virus
Global goals (SDG)
SDG3 Good health and well-being
UKÄ Subject classification
Biochemistry
Molecular Biology
Publication identifier
- DOI: https://doi.org/10.1080/15257770.2020.1720234
Permanent link to this page (URI)
https://res.slu.se/id/publ/105212