Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode

Abstract

A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0. 1 M NaCl and a concentration of gramicidin A in the range 0-12nM was used. Electrochemical impedance spectroscopy in the frequency range 0.1-65 kHz was employed to investigate how the defect-free monolayer responds to interactions of gramicidin A in solution.The data was analyzed both with multivariate data analysis and classical electrochemical methods. The principal component analysis of the resulting impedance spectra gave a linear dependence on the concentration of gramicidin A. An increasing permittivity was observed in the low-frequency regime with increasing concentration of gramicidin A in solution.

Keywords

phospholipid monolayer; gramicidin A; DOPC; impedance spectroscopy; multivariate analysis

Published in

Analytical and Bioanalytical Chemistry
2003, volume: 375, number: 5, pages: 350-355
Publisher: SPRINGER-VERLAG BERLIN

SLU Authors

• Nyström, Josefina

  • Umeå University

• Geladi, Paul

  • Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Analytical Chemistry

Publication identifier

• DOI: https://doi.org/10.1007/s00216-002-1697-9

Permanent link to this page (URI)

https://res.slu.se/id/publ/1098