Melnyk, Charles
- Department of Plant Biology, Swedish University of Agricultural Sciences
Research article2021Peer reviewedOpen access
Cell-by-cell dissection of phloem development links a maturation gradient to cell specialization
Roszak, Pawel; Heo, Jung-Ok; Blob, Bernhard; Toyokura, Koichi; Sugiyama, Yuki; Balaguer, Maria Angels de Luis; Lau, Winnie W. Y.; Hamey, Fiona; Cirrone, Jacopo; Madej, Ewelina; Bouatta, Alida M.; Wang, Xin; Guichard, Marjorie; Ursache, Robertas; Tavares, Hugo; Verstaen, Kevin; Wendrich, Jos; Melnyk, Charles W.; Oda, Yoshihisa; Shasha, Dennis;
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Abstract
In the plant meristem, tissue-wide maturation gradients are coordinated with specialized cell networks to establish various developmental phases required for indeterminate growth. Here, we used single-cell transcriptomics to reconstruct the protophloem developmental trajectory from the birth of cell progenitors to terminal differentiation in the Arabidopsis thaliana root. PHLOEM EARLY DNA-BINDING-WITH-ONE-FINGER (PEAR) transcription factors mediate lineage bifurcation by activating guanosine triphosphatase signaling and prime a transcriptional differentiation program. This program is initially repressed by a meristem-wide gradient of PLETHORA transcription factors. Only the dissipation of PLETHORA gradient permits activation of the differentiation program that involves mutual inhibition of early versus late meristem regulators. Thus, for phloem development, broad maturation gradients interface with cell-type-specific transcriptional regulators to stage cellular differentiation.
Published in
Science
2021, volume: 374, number: 6575, article number: eaba5531
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
SLU Authors
UKÄ Subject classification
Developmental Biology
Publication identifier
- DOI: https://doi.org/10.1126/science.aba5531
Permanent link to this page (URI)
https://res.slu.se/id/publ/116259