H-1, C-13 and N-15 resonance assignment of backbone and IVL-methyl side chain of the S135A mutant NS3pro/NS2B protein of Dengue II virus reveals unique secondary structure features in solution

Sammanfattning

The serotype II Dengue (DENV 2) virus is the most prevalent of all four known serotypes. Herein, we present nearly complete H-1, N-15, and C-13 backbone and H-1, C-13 isoleucine, valine, and leucine methyl resonance assignment of the apo S135A catalytically inactive variant of the DENV 2 protease enzyme folded as a tandem formed between the serine protease domain NS3pro and the cofactor NS2B, as well as the secondary structure prediction of this complex based on the assigned chemical shifts using the TALOS-N software. Our results provide a solid ground for future elucidation of the structure and dynamic of the apo NS3pro/NS2B complex, key for adequate development of inhibitors, and a thorough molecular understanding of their function(s).

Nyckelord

Dengue 2 virus; NS3 protease; Flavivirus protease; NMR chemical shifts assignment; Methyl assignment; Backbone dynamics

Publicerad i

Biomolecular NMR Assignments
2022, volym: 16, nummer: 1, sidor: 135-145
Utgivare: SPRINGER

SLU författare

• Agback, Peter

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Agback, Tatiana

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Biokemi och molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/s12104-022-10071-w

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/116269