A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component

Sammanfattning

Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.

Nyckelord

autophagy; effectors; immunity; ubiquitination; xenophagy

Publicerad i

EMBO Journal
2022, volym: 41, nummer: 13, artikelnummer: e110352
Utgivare: WILEY

SLU författare

• Minina, Alyona

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Hafrén, Anders

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

Associerade SLU-program

SLU Nätverk växtskydd
AMR: Bakterier

UKÄ forskningsämne

Mikrobiologi

Publikationens identifierare

• DOI: https://doi.org/10.15252/embj.2021110352

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/117407