The Microbial Community in a Substrate of Solid-State Fermentation by Lentinula edodes: A Preliminary Study

Sammanfattning

Edible-fungal-based solid-state fermentation holds promise for sustainable food and biofuel production. Understanding the role of microbial communities in fungal substrates is crucial. Birch-based substrates were treated with autoclaving (121 degrees C, at 2 bar) or hot air pasteurization (75-100 degrees C), followed by incubation with and without shiitake (Lentinula edodes) inoculum. Mycelial growth was monitored by CO2 release and microbial biomass by phosphate-lipid fatty acid (PLFA). DNA se-quencing was used to analyze the microbial communities. Results showed successful colonization of shiitake on all substrates, regardless of pasteurization temperatures and coexisting microbes. Total microbial respiration (CO2) and PLFA biomass showed no significant differences between pasteurization regimes. However, significant microbial differences were found between shiitake-inoculated and non-inoculated treatments. DNA sequencing revealed the dominance of Phyllobacterium, Sphingomonas, and Pelomonas genera in all inoculated substrates, while non-inoculated substrates were abundant in Bacillus spp. and Paenibacillus spp. of the Firmicutes phylum. This study provides preliminary insights into the microbial community in birch-based shiitake substrates, facilitating further investi-gation of bacteria involved in shiitake mycelium growth promotion and biochemical conversion for biofuel production.

Nyckelord

edible fungi; biofuel; hot-air pasteurization; microbial composition; wood substrate; respirator incubation; biorefinery

Publicerad i

Fermentation
2023, volym: 9, nummer: 8, artikelnummer: 736
Utgivare: MDPI

SLU författare

• Eilertsen, Lill

  • Institutionen för skogens biomaterial och teknologi, Sveriges lantbruksuniversitet
    

• Hultberg, Malin

  • Institutionen för biosystem och teknologi, Sveriges lantbruksuniversitet
    

• Lee, Natuschka

  • Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
    

• Saroj, Paramjeet

  • Institutionen för skogens biomaterial och teknologi, Sveriges lantbruksuniversitet
    

• Swaine, Mark

  • Institutionen för skogens ekologi och skötsel, Sveriges lantbruksuniversitet
    

• Chen, Feng

  • Institutionen för skogens biomaterial och teknologi, Sveriges lantbruksuniversitet
    

• Xiong, Shaojun

  • Institutionen för skogens biomaterial och teknologi, Sveriges lantbruksuniversitet
    

Globala målen (SDG)

SDG2 Ingen hunger
SDG12 Hållbar konsumtion och produktion

UKÄ forskningsämne

Mikrobiologi
Livsmedelsvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.3390/fermentation9080736

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/124938