Manell, Elin
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Columbia University
Forskningsartikel2024Vetenskapligt granskadÖppen tillgång
Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen
Hong, Julie S.; Shamim, Abrar; Atta, Hussein; Nonnecke, Eric B.; Merl, Sarah; Patwardhan, Satyajit; Manell, Elin; Gunes, Esad; Jordache, Philip; Chen, Bryan; Lu, Wuyuan; Shen, Bo; Dionigi, Beatrice; Kiran, Ravi P.; Sykes, Megan; Zorn, Emmanuel; Bevins, Charles L.; Weiner, Joshua
Sammanfattning
Intestinal transplantation is the definitive treatment for intestinal failure. However, tissue rejection and graft versus-host disease are relatively common complications, necessitating aggressive immunosuppression that can itself pose further complications. Tracking intraluminal markers in ileal effluent from standard ileostomies may present a noninvasive and sensitive way to detect developing pathology within the intestinal graft. This would be an improvement compared to current assessments, which are limited by poor sensitivity and specificity, contributing to under or over-immunosuppression, respectively, and by the need for invasive biopsies. Herein, we report an approach to reproducibly analyze ileal fluid obtained through stoma sampling for antimicrobial peptide/protein concentrations, reasoning that these molecules may provide an assessment of intestinal homeostasis and levels of intestinal inflammation over time. Concentrations of lysozyme (LYZ), myeloperoxidase (MPO), calprotectin (S100A8/A9) and beta-defensin 2 (DEFB2) were assessed using adaptations of commercially available enzyme-linked immunosorbent assays (ELISAs). The concentration of alpha-defensin 5 (DEFA5) was assessed using a newly developed sandwich ELISA. Our data support that with proper preparation of ileal effluent specimens, precise and replicable determination of antimicrobial peptide/protein concentrations can be achieved for each of these target molecules via ELISA. This approach may prove to be reliable as a clinically useful assessment of intestinal homeostasis over time for patients with ileostomies.
Nyckelord
Intestinal transplantation; Transplantation immunology; ELISA; Methods; Crohn 's disease
Publicerad i
Journal of Immunological Methods
2024, volym: 525, artikelnummer: 113599
Utgivare: ELSEVIER
SLU författare
Globala målen (SDG)
SDG3 God hälsa och välbefinnande
UKÄ forskningsämne
Immunologi inom det medicinska området
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.jim.2023.113599
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/128208