A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli

Sammanfattning

Reactive oxygen species (ROS) are important signaling agents in plants and animals. They are involved in diverse processes, including activation of immune responses to pathogen infection. Biphasic detection of ROS in response to pathogen perception is becoming more popular even in important crops like potato as means of screening different germ plasms and mutants generated by for example CRISPR-Cas9 as well as identifying signaling pathways. Here we describe a detailed protocol for quantifying ROS bursts induced in potato leaf discs in response to a bacterial elicitor and Phytophthora infestans.

Nyckelord

flg22; Immunity; Luminol; Oxidative burst; Potato; Reactive oxygen species; ROS

Publicerad i

Methods in Molecular Biology
2022, volym: 2536, sidor: 395-402
Titel: Plant Pathology : Method and Protocols
Utgivare: Springer

SLU författare

• Zahid, Muhammad Awais

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

• Vetukuri, Ramesh

  • Institutionen för växtförädling, Sveriges lantbruksuniversitet
    

• Andreasson, Erik

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Botanik
Mikrobiologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/978-1-0716-2517-0_22
• ISBN: 978-1-0716-2516-3
• eISBN: 978-1-0716-2517-0

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/129719