Differences in RAD51 transcriptional response and cell cycle dynamics reveal varying sensitivity to DNA damage among Arabidopsis thaliana root cell types

Sammanfattning

Throughout their lifecycle, plants are subjected to DNA damage from various sources, both environmental and endogenous. Investigating the mechanisms of the DNA damage response (DDR) is essential to unravel how plants adapt to the changing environment, which can induce varying amounts of DNA damage. Using a combination of whole-mount single-molecule RNA fluorescence in situ hybridization (WM-smFISH) and plant cell cycle reporter lines, we investigated the transcriptional activation of a key homologous recombination (HR) gene, RAD51, in response to increasing amounts of DNA damage in Arabidopsis thaliana roots. The results uncover consistent variations in RAD51 transcriptional response and cell cycle arrest among distinct cell types and developmental zones. Furthermore, we demonstrate that DNA damage induced by genotoxic stress results in RAD51 transcription throughout the whole cell cycle, dissociating its traditional link with S/G2 phases. This work advances the current comprehension of DNA damage response in plants by demonstrating quantitative differences in DDR activation. In addition, it reveals new associations with the cell cycle and cell types, providing crucial insights for further studies of the broader response mechanisms in plants.

Nyckelord

Arabidopsis thaliana; cell cycle arrest; cell cycle checkpoints; DNA damage; double-strand breaks; homologous recombination; RAD51 transcription

Publicerad i

New Phytologist
2024, volym: 243, nummer: 3, sidor: 966-980
Utgivare: WILEY

SLU författare

• Kutashev, Konstantin

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Fonseca Cárdenas, Alejandro

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Sartori, Kevin

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Sicard, Adrien

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Rosa, Stefanie

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Botanik

Publikationens identifierare

• DOI: https://doi.org/10.1111/nph.19875

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/130898