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Bokkapitel2023Vetenskapligt granskad

Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurements

Omar-Hmeadi, M.; Liu, L.; Echeverry, S.; Barg, S.

Sammanfattning

Hormones and neurotransmitters are released from (neuro)endocrine cells by regulated exocytosis of secretory granules. During exocytosis, the granule membrane fuses with the plasma membrane, which allows release of the stored content into the bloodstream or the surrounding tissue. Here, we give a detailed description of two complementary methods to observe and quantify exocytosis in single cells: high-resolution TIRF microscopy and patch-clamp capacitance recordings. Precise stimulation of exocytosis is achieved by local pressure application or voltage-clamp depolarizations. While the chapter is focused on insulin-secreting cells as an accessible and disease-relevant model system, the methodology is applicable to a wide variety of secretory cells including chromaffin and PC12 cells.

Nyckelord

Chromaffin cells; Insulin release; Patch-clamp; PC12; Secretory vesicles

Publicerad i

Methods in Molecular Biology
2023, volym: 2565, nummer: 2565, sidor: 179-186
Titel: Methods in Molecular Biology : Methods and Protocols
Utgivare: Springer

SLU författare

UKÄ forskningsämne

Cell- och molekylärbiologi

Publikationens identifierare

  • DOI: https://doi.org/10.1007/978-1-0716-2671-9_12
  • ISBN: 978-1-0716-2670-2
  • eISBN: 978-1-0716-2671-9

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/131978