Mamani, Marisel
- Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
- Universidad Mayor de San Andres
Sammanfattning
Trichoderma fungi are colonizers of plant substrates and rhizosphere and are valued for their antagonism against phytopathogens and ability to promote plant health. We investigated Trichoderma diversity in coffee-growing soils in Caranavi region of Yungas-La Paz, Bolivia, where high humidity and fungal diseases threaten yield, and evaluated their potential as biocontrol agents against coffee pathogens. A total of 440 Trichoderma were isolated from coffee rhizosphere, fallow lands, and forest ecosystems across an altitudinal gradient in Caranavi. DNA barcode analyses using ITS, rpb2, and tef1 loci identified only four species. However, 47 taxa comprising 344 isolates were ambiguous, and 41 isolates were previously unrecognised species. The diversity of Trichoderma spp. was significantly affected by ecosystem type and altitude, with more species isolated from coffee rhizosphere than fallow lands and forest ecosystems, and from lower altitudes than higher ones. Evaluation of 100 isolates against a native coffee wilt pathogen Fusarium oxysporum identified 70 potent antagonists, with 30 achieving 90-100% disease control. This is the first comprehensive study of Trichoderma diversity in Yungas, identifying indigenous Trichoderma for biocontrol applications against coffee diseases. It also emphasizes the need to refine the Trichoderma species concept and improve the taxonomic resolution within the genus.An investigation of Trichoderma diversity in the unexplored Amazonic region of Bolivia highlights the importance of refining the Trichoderma species concept and improving taxonomic resolution within the genus.
Nyckelord
biofungicide; Caranavi-Yungas; coffea arabica; coffee rhizosphere; DNA barcode; disease management; fungal diversity; intraspecies variation
Publicerad i
FEMS Microbiology Letters
2026, volym: 373, artikelnummer: fnaf142
Utgivare: OXFORD UNIV PRESS
SLU författare
UKÄ forskningsämne
Mikrobiologi
Publikationens identifierare
- DOI: https://doi.org/10.1093/femsle/fnaf142
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/145809