Roy, Shweta
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Sammanfattning
Potato mop-top virus (PMTV) is a significant pathogen causing potato "spraing" disease worldwide. The PMTV 8K protein functions as a weak viral suppressor of RNA silencing (VSR), has viroporin activity, and plays a role in pathogenicity by promoting viral long-distance movement and modulating host responses. Uniquely, PMTV can establish systemic infection in the absence of the 8K protein, though the infection is slightly delayed. To elucidate the molecular mechanisms underlying PMTV-host interactions, we conducted comprehensive RNA-seq analysis comparing wild-type PMTV with a mutant lacking the 8K gene (PMTV-Delta 8K) in Nicotiana benthamiana. Our transcriptomic analysis shows that wild-type PMTV and PMTV-Delta 8K elicit largely distinct transcriptional responses in the host, with more unique than shared differentially expressed genes. The analysis also revealed extensive reprogramming of metabolic pathways, stress responses, and defense mechanisms. Notably, wild-type PMTV induced more defense-related transcription factors, including 27 WRKY genes compared with 8 in PMTV-Delta 8K infections. RNA-silencing pathway genes displayed distinct expression patterns, with AGO2, RDR1, and AGO-MEL1 showing notably enhanced upregulation (up to 9.7-fold) in PMTV-Delta 8K infections. Functional analysis identified chloroplast-associated genes GNS2, CHUP1, and KIN5l as host restriction factors. Virus-induced gene silencing experiments confirmed that GNS2 and CHUP1 restrict viral accumulation under both infection scenarios (wild-type PMTV and PMTV-Delta 8K), and localization studies revealed that the TGB2 protein and GNS2 colocalize at chloroplast structures. These findings provide insights into PMTV pathogenesis, suggest that 8K is a multifunctional protein operating through diverse mechanisms, and advance understanding of viral suppression strategies.Copyright (c) 2026 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Nyckelord
potato mop-top virus; RNA-seq; VIGS
Publicerad i
Molecular plant-microbe interactions
2026
Utgivare: AMER PHYTOPATHOLOGICAL SOC
SLU författare
Saripella, Ganapathi Varma
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
Vetukuri, Ramesh
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
Siddique, Abu Bakar
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Savenkov, Eugene
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Jordbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1094/MPMI-10-25-0146-R
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/146670