Heterologous array analysis in Heterobasidion: Hybridisation of cDNA arrays with probe from mycelium of S, P or F-types

Sammanfattning

Because of the close relatedness between three species of Heterobasidion annosum (P type), Heterobasidion parviporum (S-type) and Heterobasidion abietinum (F-type). we investigated the possible use of arrays from one species for studies of gene expression in the other. Clones containing partial cDNAs from 94 identifiable genes expressed during spore germination and differentiation in H. parviporum were printed manually in six replications on nylon membranes. The membrane was hybridized with chemifluorescent labelled cDNA from actively growing mycelia of H. parviporum, H. annosum or H. abietinum, cultivated on a non-selective substrate. Product-moment correlation coefficient varied between 0.81 and 0.49. Due to the level of correlation, in the gene expression among the intersterility groups, we concluded that the cDNA array of one can be used to study gene expression in the others. (C) 2008 Elsevier B.V. All rights reserved.

Nyckelord

Heterobasidion annosum; Heterobasidion parviporum; Heterobasidion abietinum; Gene expression; Conservation; Macroarray

Publicerad i

Journal of Microbiological Methods
2008, volym: 75, nummer: 2, sidor: 219-224
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Lundén, Karl

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Finlay, Roger

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Stenlid, Jan

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Asiegbu, Frederick

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    
  • Helsingin yliopisto

UKÄ forskningsämne

Miljö- och naturvårdsvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.mimet.2008.06.014

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/18730