Snf1-related protein kinase 1 is needed for growth in a normal day-night light cycle

Abstract

The yeast Snf1 protein kinase and its animal homologue, the AMP-activated protein kinase, play important roles in metabolic regulation, by serving as energy gauges that turn off energy-consuming processes and mobilize energy reserves during low-energy conditions. The closest homologue of these kinases in plants is Snf1-related protein kinase 1 (SnRK1). We have cloned two SnRK1-encoding genes, PpSNF1a and PpSNF1b, in the moss Physcomitrella patens, where gene function can be studied directly by gene targeting in the haploid gametophyte. A snf1a snf1b double knockout mutant is viable, but lacks all Snf1-like protein kinase activity. The mutant has a complex phenotype that includes developmental abnormalities, premature senescence and altered sensitivities to plant hormones. Remarkably, the double knockout mutant also requires continuous light, and is unable to grow in a normal day-night light cycle. This suggests that SnRK1 is needed for metabolic changes that help the plant cope with the dark hours of the night

Published in

EMBO Journal
2004, volume: 23, number: 8, pages: 1900-1910
Publisher: NATURE PUBLISHING GROUP

SLU Authors

• Thelander, Mattias

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Ronne, Hans

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Olsson, Tina

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1038/sj.emboj.7600182

Permanent link to this page (URI)

https://res.slu.se/id/publ/2308