Forskningsartikel2012Vetenskapligt granskadÖppen tillgång
Redox-mediated Mechanisms Regulate DNA Binding Activity of the G-group of Basic Region Leucine Zipper (bZIP) Transcription Factors in Arabidopsis
Shaikhali, Jehad; Norén, Louise; Barajas-López, Juan de Dios; Srivastava, Vaibhav; König, Janine; Sauer, Uwe H.; Wingsle, Gunnar; Dietz, Karl-Josef; Strand, Åsa
Sammanfattning
Plant genes that contain the G-box in their promoters are responsive to a variety of environmental stimuli. Bioinformatics analysis of transcriptome data revealed that the G-box element is significantly enriched in promoters of high light-responsive genes. From nuclear extracts of high light-treated Arabidopsis plants, we identified the AtbZIP16 transcription factor as a component binding to the G-box-containing promoter fragment of light-harvesting chlorophyll a/b-binding protein2.4 (LHCB2.4). AtbZIP16 belongs to the G-group of Arabidopsis basic region leucine zipper (bZIP) type transcription factors. Although AtbZIP16 and its close homologues AtbZIP68 and AtGBF1 bind the G-box, they do not bind the mutated half-sites of the G-box palindrome. In addition, AtbZIP16 interacts with AtbZIP68 and AtGBF1 in the yeast two-hybrid system. A conserved Cys residue was shown to be necessary for redox regulation and enhancement of DNA binding activity in all three proteins. Furthermore, transgenic Arabidopsis lines overexpressing the wild type version of bZIP16 and T-DNA insertion mutants for bZIP68 and GBF1 demonstrated impaired regulation of LHCB2.4 expression. Finally, overexpression lines for the mutated Cys variant of bZIP16 provided support for the biological significance of Cys330 in redox regulation of gene expression. Thus, our results suggest that environmentally induced changes in the redox state regulate the activity of members of the G-group of bZIP transcription factors.
Publicerad i
Journal of Biological Chemistry
2012, volym: 287, nummer: 33, sidor: 27510-27525
Utgivare: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
SLU författare
Srivastava, Vaibhav
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
Wingsle, Gunnar
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
Strand, Åsa
- Umeå Universitet
UKÄ forskningsämne
Biokemi och molekylärbiologi
Publikationens identifierare
- DOI: https://doi.org/10.1074/jbc.M112.361394
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/45151