Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Sammanfattning

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.

Nyckelord

Whole blood; RNA preservation; RNA extraction; DNA contamination; Transcriptome sequencing; Globin transcripts

Publicerad i

BMC Biotechnology
2012, volym: 12, sidor: 1-11
Utgivare: BIOMED CENTRAL LTD

SLU författare

• Schowochow, Doreen

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Mikrobiologi
Annan miljöbioteknik

Publikationens identifierare

• DOI: https://doi.org/10.1186/1472-6750-12-33

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/45166