5 '-Deoxyisopentenyladenosine and other cytokinins in culture filtrates of the bacterium Pantoea agglomerans

Abstract

A Pantoea agglomerans isolate from barley seeds, selected for inducing growth promotion in tomato test plants, was found to excrete several hormones of the cytokinin class into its culture medium. In addition to isopentenyladenine, isopentenyladenosine and the 2-methylthiol derivates of these, an unknown compound with affinity to anticytokinin antibodies was also isolated. Mass spectroscopy indicated the structure of this to be a deoxyisopentenyladenosine. The structure of 9-(5'-deoxy-beta-D-ribofuranosyl)-6-(3-methyl-2-butenylamino)purine was verified after synthesis of standards and analysis with GC-MS. The synthesized 5'-deoxyisopentenyl-adenosine showed activity in the Amaranthus bioassay, specific for cytokinins. To our knowledge this is the first report of a naturally occurring cytokinin containing 5'-deoxyribose

Published in

Physiologia Plantarum
2004, volume: 121, number: 3, pages: 439-447
Publisher: BLACKWELL MUNKSGAARD

SLU Authors

• Nicander, Björn

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Gerhardson, Berndt

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Landscape Architecture
Environmental Sciences related to Agriculture and Land-use
Food Science

Publication identifier

• DOI: https://doi.org/10.1111/j.1399-3054.2004.00337.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/4715