Evaluation of Pollen Contamination in an Advanced Scots Pine Seed Orchard

Abstract

The pollination pattern in a Scots pine (Pinus sylvestris L.) seed orchard consisting of 28 clones was studied using nine microsatellite (SSR) loci. The nine SSR loci produced unique multilocus genotypes for each of the orchard's 28 clones and allowed paternal assignment of the studied 305 seed using paternity exclusion probability of 99.9%. Fifty two percent of the studied seeds were sired by outside the orchard pollen sources (i.e., pollen contamination) and as expected, low selfing (2.3%) was detected. These results are valuable for the evaluation of the seed orchard function and the impact of contamination on the expected genetic gain.

Keywords

Paternity analysis; Pinus sylvestris; pollination; SSR marker

Published in

Silvae Genetica
2009, volume: 58, number: 5-6, pages: 262-269
Publisher: J D SAUERLANDERS VERLAG

SLU Authors

• Fries, Anders

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Lindgren, Dag

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Publication identifier

• DOI: https://doi.org/10.1515/sg-2009-0033

Permanent link to this page (URI)

https://res.slu.se/id/publ/48996