The effect of combining linseed oil and sesamin on the fatty acid composition in white muscle and on expression of lipid-related genes in white muscle and liver of rainbow trout (Oncorhynchus mykiss)

Abstract

Sesamin (S) is a known lipid modulator and has been shown to increase the conversion of alpha-linolenic acid (ALA) to docosahexaenoic acid in rainbow trout (Oncorhynchus mykiss) fed vegetable oil mixtures including linseed oil. In this study, we evaluated the effects of S supplementation in linseed oil-based diets, content of alpha- and gamma-tocopherols, fatty acid (FA) composition, as well as the gene expression of lipid-related genes. Fish with an average weight of 36.5 g were fed different combinations of commercial linseed oil (LO), purified linseed oil triacylglycerols (TAG) with polar fraction removed and a mixed linseed-sunflower oil (6:4 v/v) (MO). S was added at 0.58 g 100(-1)g feed and fed to the fish for a period of 58 days. Expression of PPAR alpha was downregulated in white muscle of fish fed S containing diets (P < 0.05). The expression of PPAR beta 1A was not affected by S supplementation except where TAG oil was used. The expression of PPAR beta 1A declined significantly in TAG + S fed group (P < 0.05), which indicates that some minor compounds in linseed oil might suppress the effect of S on the expression of PPAR beta 1A. The expression of PPAR gamma(long) declined in LO + S and MO + S fed group (P < 0.05). The beta-oxidation-related genes CPT1 and ACO were upregulated by vegetable oils compared to fish oil. S decreased percentage of ALA in white muscle of fish fed LO + S (P < 0.05). The increased desaturation index and the decreased ALA levels suggest that S may increase the biosynthesis of highly unsaturated FA in rainbow trout.

Keywords

ACO; ALA; CPT1; Delta 6 fatty acid desaturase; DHA; Linseed oil; PPARs; Sesamin; Sunflower oil

Published in

Aquaculture International
2013, volume: 21, number: 4, pages: 843-859
Publisher: SPRINGER

SLU Authors

• Pan, Jinfeng

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Schiller Vestergren, Anna

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Trattner, Sofia

  • Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
    

• Johnsson, Pernilla

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Kamal-Eldin, Afaf

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Brännäs, Eva

  • Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
    

• Pickova, Jana

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Moazzami, Ali

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology
Cell Biology
Fish and Aquacultural Science

Publication identifier

• DOI: https://doi.org/10.1007/s10499-012-9511-y

Permanent link to this page (URI)

https://res.slu.se/id/publ/55288