An AFLP-markers based genetic linkage map of Heterobasidion annosum locating intersterility genes

Abstract

A genetic linkage map of the basidiomycete Heterobasidion annosum, casual agent of root rot in conifers, was constructed from a compatible mating between isolates from the North American S and P intersterifity groups. In a population consisting of 102 progeny isolates, 358 AFLP markers were scored. The linkage analysis generated 19 large linkage groups, containing 6 or more markers, which covered 1468 cM. The physical size to genetic distance was approximately 11.1 kbp/cM. Segregation of three intersterility gene loci were analysed through mating of the progeny isolates with three tester strains carrying known intersterility genotypes. The loci for the two intersterility genes (S and P) were successfully located in the map. Segregation of the mating type locus was analysed by backcrossing the progeny isolates with their parental strains. The mating type locus could not be located in the map. (c) 2005 Elsevier Inc. All rights reserved

Keywords

Heterobasidion annosum; Genetic linkage map; Inersterility genes; AFLP markers; Forest pathogen; Mating type

Published in

Fungal Genetics and Biology
2005, volume: 42, number: 6, pages: 519-527
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU Authors

• Lind, Mårten

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Olson, Åke

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Stenlid, Jan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences related to Agriculture and Land-use

Publication identifier

• DOI: https://doi.org/10.1016/j.fgb.2005.03.005

Permanent link to this page (URI)

https://res.slu.se/id/publ/5823