Development of a genetic transformation method for seabuckthorn ( Hippophae rhamnoides L.).

Sammanfattning

Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing highamounts of several bioactive compounds with nutritional and medicinal traits. It is also planted tocontrol soil erosion. A genetic transformation procedure will facilitate studies of the control ofplant development and interactions with symbionts and pathogens, and will provide a tool forplant breeding. Here, we present a particle bombardment method for transforming seabuckthorn.The early stages of induced adventitious shoots from roots were chosen as a novel target tissue forthe transformation procedure. The root system was bombarded with gold particles coated withplasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,and shoots were regenerated under kanamycin selection. PCR analysis of the regeneratedtransformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kbfragment from the 35S promoter in three shoots from independent transformation events.

Publicerad i

American Journal of Plant Sciences
2014, volym: 5, nummer: 5, sidor: 528-534

SLU författare

• Sriskandarajah, Sridevy

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Clapham, David

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Lundquist, Per-Olof

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Växtbioteknologi

Publikationens identifierare

• DOI: https://doi.org/10.4236/ajps.2014.55067

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/66853