Using a Reverse Genetics Approach to Investigate Small-Molecule Activity

Sammanfattning

Chemical genomics is a highly effective approach for understanding complex and dynamic biologicalprocesses in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a hugeabundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here wepresent an approach to characterize a chemical of interest, as well as examples of studies demonstrating aneffective combination of chemical genomics with reverse genetics strategies, drawn from recent literatureon phytohormone signalling and auxin transport.

Nyckelord

Chemical genomics; Arabidopsis mutants; Reverse genetics; Phytohormone signalling; Auxin transport

Publicerad i

Methods in Molecular Biology
2014, volym: 1056, nummer: 1056, sidor: 51-62
Titel: Plant chemical genomics : methods and protocols
Utgivare: Springer

SLU författare

• Doyle, Siamsa

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Robert, Stephanie

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Biokemi och molekylärbiologi
Botanik
Utvecklingsbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/978-1-62703-592-7_6
• ISBN: 978-1-62703-591-0
• eISBN: 978-1-62703-592-7

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/79515