Microbial community structure and activity in trace element-contaminated soils phytomanaged by Gentle Remediation Options (GRO)

Sammanfattning

Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe. Community structure was studied using denaturing gradient gel electrophoresis (DGGE) fingerprinting of Bacteria, alpha- and beta-Proteobacteria, Actinobacteria and Streptomycetaceae, and sequencing of DGGE bands characteristic of specific treatments. The number of copies of genes involved in ammonia oxidation and denitrification were determined by qPCR.Phytomanagement increased soil microbial biomass at three sites and respiration at the Biogeco site (France). Enzyme activities were consistently higher in treated soils compared to untreated soils at the Biogeco site. At this site, microbial biomass increased from 696 to 2352 mg ATP kg(-1) soil, respiration increased from 7.4 to 40.1 mg C-CO2 kg(-1) soil d(-1), and enzyme activities were 2-11-fold higher in treated soils compared to untreated soil. Phytomanagement induced shifts in the bacterial community structure at both, the total community and functional group levels, and generally increased the number of copies of genes involved in the N cycle (nirK, nirS, nosZ, and amoA). The influence of the main soil physico-chemical properties and trace element availability were assessed and eventual site-specific effects elucidated. Overall, our results demonstrate that phytomanagement of TECS influences soil biological activity in the long term. (C) 2017 Elsevier Ltd. All rights reserved.

Nyckelord

Microbial community structure; Enzyme activity; Phytoexclusion; Phytoextraction; Phytostabilization

Publicerad i

Environmental Pollution
2017, volym: 231, nummer: Part 1, sidor: 237-251

SLU författare

• Dimitriou, Ioannis

  • Institutionen för växtproduktionsekologi, Sveriges lantbruksuniversitet
    

Associerade SLU-program

SLU Nätverk växtskydd

Globala målen (SDG)

SDG15 Ekosystem och biologisk mångfald

UKÄ forskningsämne

Annan geovetenskap och miljövetenskap
Miljövetenskap
Annan biologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.envpol.2017.07.097

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/87630