A standardized toolkit for genetic engineering of CTG Glade yeasts

Defosse, Tatiana A.; Courdavault, Vincent; Coste, Alix T.; Clastre, Marc; de Bernonville, Thomas Duge; Godon, Charlotte; Vandeputte, Patrick; Lanoue, Arnaud; Touze, Antoine; Linder, Tomas; Droby, Samir; Rosa, Carlos A.; Sanglard, Dominique; d'Enfert, Christophe; Bouchara, Jean-Philippe; Giglioli-Guivarc'h, Nathalie; Papon, Nicolas

doi:10.1016/j.mimet.2017.11.015

Research article2018Peer reviewed

A standardized toolkit for genetic engineering of CTG Glade yeasts

Defosse, Tatiana A.; Courdavault, Vincent; Coste, Alix T.; Clastre, Marc; de Bernonville, Thomas Duge; Godon, Charlotte; Vandeputte, Patrick; Lanoue, Arnaud; Touze, Antoine; Linder, Tomas; Droby, Samir; Rosa, Carlos A.; Sanglard, Dominique; d'Enfert, Christophe; Bouchara, Jean-Philippe; Giglioli-Guivarc'h, Nathalie; Papon, Nicolas

Abstract

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG Glade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.

Keywords

Candida; Synthetic construct; Standardization; beta-galactosidase; Fluorescent proteins; Luciferase

Published in

Journal of Microbiological Methods
2018, volume: 144, pages: 152-156

SLU Authors

Linder, Tomas
- Department of Molecular Sciences, Swedish University of Agricultural Sciences

UKÄ Subject classification

Genetics
Microbiology

Publication identifier

DOI: https://doi.org/10.1016/j.mimet.2017.11.015

Permanent link to this page (URI)

https://res.slu.se/id/publ/92152