Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis thaliana

Abstract

Plants have a remarkable ability to regenerate vascular tissue after damage or wounding. A striking example of this phenomenon is the cutting and rejoining of plants during the process of grafting, which humans have used for millennia. Here, I describe how to graft Arabidopsis seedlings and how to monitor the vascular reconnection process during wound healing using fluorescent dyes and fluorescent proteins. Furthermore, I describe how to visualize xylem formation during graft healing. These techniques are useful for studying how the vascular system regenerates and for better understanding the process of plant grafting.

Keywords

Arabidopsis thaliana; Micro-grafting; Chimeric plants; Fluorescent dyes; Vascular regeneration; Xylem formation

Published in

Methods in Molecular Biology
2017, volume: 1544, number: 1544, pages: 91-102
Title: Xylem: Methods and Protocols
Publisher: Springer

SLU Authors

• Melnyk, Charles

  • University of Cambridge

UKÄ Subject classification

Developmental Biology

Publication identifier

• DOI: https://doi.org/10.1007/978-1-4939-6722-3_9
• ISBN: 978-1-4939-6722-3
• eISBN: 978-1-4939-6720-9

Permanent link to this page (URI)

https://res.slu.se/id/publ/94468