Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis

Abstract

Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (100 samples d(-1)) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.

Keywords

Arabidopsis thaliana; auxin; metabolite profiling; multivariate data analysis; mutant; screening

Published in

Journal of Experimental Botany
2018, volume: 69, number: 10, pages: 2569-2579
Publisher: OXFORD UNIV PRESS

SLU Authors

• Pencik, Ales

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Institute of Experimental Botany ASCR
  • Palacký University Olomouc

• Casanova, Ruben

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Ljung, Karin

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Novak, Ondrej

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Institute of Experimental Botany ASCR
  • Palacký University Olomouc

UKÄ Subject classification

Botany

Publication identifier

• DOI: https://doi.org/10.1093/jxb/ery084

Permanent link to this page (URI)

https://res.slu.se/id/publ/95277