Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics

Sammanfattning

Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.

Publicerad i

Plant Physiology
2018, volym: 177, nummer: 2, sidor: 476-489
Utgivare: AMER SOC PLANT BIOLOGISTS

SLU författare

• Simura, Jan

  • Akademie věd České republiky

• Antoniadi, Ioanna

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Ljung, Karin

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Novak, Ondrej

  • Akademie věd České republiky

UKÄ forskningsämne

Botanik

Publikationens identifierare

• DOI: https://doi.org/10.1104/pp.18.00293

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/95887