Lipid profile of bovine blastocysts exposed to insulin during in vitro oocyte maturation

Sammanfattning

Insulin is a key hormone with important functions in energy metabolism and is involved in the regulation of reproduction. Hyperinsulinaemia is known to impair fertility (for example, in obese mothers); therefore, we aimed to investigate the impact of elevated insulin concentrations during the sensitive period of oocyte maturation on gene expression and lipid profiles of the bovine Day-8 embryo. Two different insulin concentrations were used during in vitro oocyte maturation (INS10 = 10 mu g mL(-1) and INS0.1 = 0.1 mu g mL(-1)) in order to observe possible dose-dependent effects or thresholds for hyperinsulinaemia in vitro. By investigating gene expression patterns by an mRNA microarray in combination with lipid profile analysis by desorption electrospray ionisation-mass spectrometry (DESI-MS) of embryos derived from insulin-treated oocytes, we gained further insights regarding molecular responses of embryos to insulin provocation during the first days of development. Lipid metabolism appeared to be influenced on multiple levels according to gene expression results but the profiles collected in positive-ion mode by DESI-MS (showing mostly ubiquinone, cholesteryl esters and triacylglycerols) did not differ significantly from controls. There are parallels in follicular development of ruminants and humans that make this bovine model relevant for comparative research on early human embryonic development during hyperinsulinaemia.

Nyckelord

early embryonic development; hyperinsulinaemia; lipid metabolism; metabolic imbalance

Publicerad i

Reproduction, Fertility and Development
2018, volym: 30, nummer: 9, sidor: 1253-1266
Utgivare: CSIRO PUBLISHING

SLU författare

• Laskowski, Denise

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Andersson, Göran

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Humblot, Patrice

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Sjunnesson, Ylva

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Båge, Renee

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Klinisk vetenskap
Husdjursvetenskap
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Publikationens identifierare

• DOI: https://doi.org/10.1071/RD17248

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/96393