Sandström, Corine
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
(Glucurono)arabinoxylans were extracted from barley husks and degraded with endo-beta-xylanase or subjected to periodate oxidation. The released oligosaccharide fragments were separated and isolated on Biogel-P2, and their structures were determined by NMR spectroscopy. The oligosaccharides identified consisted Of beta-D-(1-4)-linked xylopyranosyl residues, of which some were substituted at O-3 with alpha-L-arabinofuranosyl groups or at O-2 with 4-0-methylglucuronic acid. In addition to these substituents, a disaccharide side chain, 2-O-beta-D-Xylopyranosyl-alpha-L-arabinofuranose, attached at position O-3 of the main chain, was proved to exist in arabinoxylan from barley husks. The compound was fully characterized with NMR, and all H-1 and C-13 NMR signals were assigned. The arabinose to xylose ratio was low (similar to 0.2) and no 2,3 -disubstitution existed. No blocks of substituted xylose residues could be observed along the main chain. (c) 2006 Elsevier Ltd. All rights reserved.
Keywords
barley husk; arabinoxylan; structure; NMR spectroscopy; periodate oxidation; enzymatic hydrolysis
Published in
Carbohydrate Research
2006, volume: 341, number: 18, pages: 2959-2966
SLU Authors
Andersson, Roger
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Gohil, Suresh
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Publication identifier
- DOI: https://doi.org/10.1016/j.carres.2006.10.008
Permanent link to this page (URI)
https://res.slu.se/id/publ/9794