High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana

Abstract

We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.

Keywords

Cytokinins; Fluorescence-activated cell sorting (FACS); Solid-phase extraction (SPE); Liquid chromatography-mass spectrometry (LC-MS); Roots; Arabidopsis; Protoplasts

Published in

Methods in Molecular Biology
2017, volume: 1497, number: 1497, pages: 231-248
Title: Plant Hormones
Publisher: Humana Press

SLU Authors

• Novak, Ondrej

  • Palacký University Olomouc

• Antoniadi, Ioanna

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Ljung, Karin

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology

Publication identifier

• DOI: https://doi.org/10.1007/978-1-4939-6469-7_1
• ISBN: 978-1-4939-6467-3
• eISBN: 978-1-4939-6469-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/87058