High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana

Sammanfattning

We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.

Nyckelord

Cytokinins; Fluorescence-activated cell sorting (FACS); Solid-phase extraction (SPE); Liquid chromatography-mass spectrometry (LC-MS); Roots; Arabidopsis; Protoplasts

Publicerad i

Methods in Molecular Biology
2017, volym: 1497, nummer: 1497, sidor: 231-248
Titel: Plant Hormones
Utgivare: Humana Press

SLU författare

• Novak, Ondrej

  • Univerzity Palackého v Olomouci

• Antoniadi, Ioanna

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Ljung, Karin

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Cellbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/978-1-4939-6469-7_1
• ISBN: 978-1-4939-6467-3
• eISBN: 978-1-4939-6469-7

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/87058